WE DISCOVER INNOVATIVE DRUGS FOR SKELETAL MUSCLE PRESERVATION
using groundbreaking muscle modelling technology
Harnessing ProFuse’s extensive expertise in skeletal muscle growth, we have developed a cutting-edge in vitro high-throughput drug discovery platform for skeletal muscle loss and metabolic diseases, that enables precise and efficient screening of novel therapeutics with high translational potential.
Muscle Atrophy Models
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Biologically relevant for various indications
Obesity, sarcopenia and cachexia.
High-throughput Screening
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Efficient & predictive
- Molecules from drug discovery libraries.
- Al target-based designed molecules.
Functional Validation
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Proprietary 2D/3D assays
Enhanced translational potential.
The platform incorporates:
- A robust cell insult assay for the induction of muscle atrophy, facilitating the identification of potential therapeutic interventions.
- A high-throughput drug screening system enables efficient evaluation of candidate compounds.
- 2D and 3D assays providing accurate validation of muscle function.
- Proprietary, human muscle reporter cell lines, enabling continuous and precise assessment of skeletal muscle condition.
- In-house real-time microscopy image analysis platform with high throughput 2D and 3D image analysis.
Drug discovery strategies
- Generation of drug candidates for innovative targets using AI technology.
- High throughput screening of discovery libraries.
ProFuse drug screening advantages:
ProFuse’s technology enables rapid, consistent, and scalable growth of fully mature human muscle fibers in vitro in 2D and 3D environments, facilitating the modeling of a more efficient and predictive model of muscle atrophy for drug evaluation. This provides extensive information on drug effects, including phenotypic aspects and underlying biology, and is highly scalable for high-throughput and high-content screening.
ProFuse will have the first reliable in vitro high throughput screening system for drug and target discovery for muscle atrophy.
Advantages of the platform include:
- Rapid growth of fully mature muscle fibers.
- Consistency in tissue production.
- Scalability for high-throughput screening.
- Extensive drug effect information.
- Relevant muscle atrophy models.
- Proprietary algorithms for analysis.
The Science
ProFuse proprietary media supplements significantly accelerate differentiation, fusion, and maturation rate of muscle progenitor cells.
Differentiation, fusion and maturation: the video demonstrates the impact of the ProFuse media supplements on the fusion process of myoblast cells into newly formed skeletal muscle fibers. Synchronization and enhancement of the process lead to more mature muscle tissue with higher biological relevance.
Myogenesis: the video captures the fusion of myoblast cells into newly formed skeletal muscle fibers.
Maturation: ProFuse’s technology enables the generation of muscle tissue with mature contractile function, as indicated by the presence of organised sarcomeres. The muscle produced using ProFuse’s technology is molecularly and structurally identical to mature native muscle fibers, resulting in a superior in-vitro model.
Mature skeletal muscle fibers with sarcomeres, using ProFuse proprietary technology
The efficiency of ProFuse’s technology has been validated across various types of human myoblasts, including primary, immortalized, and iPSC derived.
Mature human iPSC derived myoblasts, using ProFuse proprietary technology
Muscle growth in 3D models: the technology enables exceptional modelling of skeletal muscle by growing fully mature and functional human muscle cells in 3D environment.
3D human Bioartificial Muscle (BAM) produced using ProFuse’s proprietary technology.
inset: A scheme of a bioartificial muscle, for tissue’s functional evaluation.
Representative data generated by the MIA proprietary platform.
Muscle Image Analysis (MIA): ProFuse has developed a proprietary image analysis platform, enabling high-throughput, accurate, and real-time data acquisition of muscle physiology and disease status in both 2D and 3D models. Key parameters analysed include fiber size, fiber maturity, and fusion index.
Proprietary human muscle cell lines expressing atrophy induced fluorescent reporters which facilitate high throughput live imaging
Reporter cell lines: ProFuse has developed proprietary cell lines expressing specific muscle maturation and atrophy reporters enabling continuous and accurate assessment of skeletal muscle health.