We are the muscle cultivation experts
Revolutionizing cultivated meat production
ProFuse develops cutting-edge spontaneously immortalized myoblast cell lines, muscle differentiation media supplements, and protocols to enhance muscle growth, increase yield, accelerate production cycles, and significantly reduce costs.The commercial viability of cultivated meat depends on overcoming cost, scaling, and quality challenges in muscle production. Current low muscle fiber conversion rates hinder industry progress. ProFuse's products optimize muscle cultivation.
Cultivated Meat Production Process
Production Scale-up Demands Optimization
Differentiation, fusion and maturation: the video demonstrates the impact of the ProFuse media supplements on the fusion process of myoblast cells into newly formed skeletal muscle fibers. Synchronization and enhancement of the process lead to more mature muscle tissue with higher biological relevance.
Myogenesis: the video captures the fusion of myoblast cells into newly formed skeletal muscle fibers.
The PROFUSE supplement is a “cookie cutter” product effective across multiple production strategies.
It works on various species, including beef, pork, lamb, chicken, and different fish species.
It has also been demonstrated to be effective on pluripotent stem cell derived myoblasts, trans-differentiated myoblasts as well as primary and immortalized myoblasts.
Additionally, it has proven effective in various 3D environments, including scaffolds, microcarriers, and suspension cultures.
The PROFUSE supplement not only increases protein content fivefold but also facilitates the formation of sarcomere structures and mature muscle proteins. This enhances both the nutritional value and the organoleptic experience.
Muscle Tissue on Scaffold (3D)
PROFUSE enhances muscle fiber formation on 3D scaffolds
ProFuse media supplements promote enhanced differentiation of muscle progenitor cells in three-dimensional cultures utilizing both edible scaffolds and microcarriers.
PROFUSE
DM+PROFUSE-S1 myosin heavy chain staining
Benchmark
Differentiation media (DM) myosin heavy chain staining
Our Muscle Cell-Line Technology
PROFUSE-B8: a Spontaneously Immortalized Bovine Myoblast Cell Line
ProFuse has developed a proprietary method for creating optimized cell lines for the efficient, industrial-scale production of nutritious and delicious muscle tissue. These spontaneously immortalized (non-GMO) cell lines are unique in as they maintain high proliferation as well as differentiation potential. Our cell lines have demonstrated consistent performance in terms of doubling time and differentiation ability for over 75 passages, both in 2D and 3D culture systems, including in serum-free media. The PROFUSE-B8 bovine cell line is available for licensing.
- Spontaneously immortalized adherent myoblast cell line (No GMO)
- In continuous culture for over 12 months
- Easily adapted to grow in serum free media
- Performance consistent over 75 passages (p25- p100)
- Both serum dependent and serum free cells have a population doubling time of approx. 20 hours
- Prospect: Chicken, pork, lamb and fish muscle cell lines
PROFUSE-S1 enhances
bovine myoblast cells
differentiation
(48 hours post treatment)
The PROFUSE-B8 cell line is now available for licensing to cultivated meat providers. Evaluation License includes:
- Evaluation period of 12m
- x2 vials of cell lines (500k cells in each)
- Up to 10 PROFUSE-S1 Vials (1mg)
- Proliferation and differentiation protocols (2D growth)
- Proliferation and differentiation protocols (3D growth)
- Media recipes
- Media for initial thaw (0.5L)
- Full cell-line datasheet
- Banking service - guarantee of resupply of same clone for the licensing term
- Access to the MIA image analysis system (2D only)
The ProFuse Roadmap
